Selected Recent Publications- Amnon Kohen

• Sen, A.; Kohen, A. Quantum Effects in Enzyme Kinetics, in Quantum Tunnelling in Enzyme Catalyzed Reactions Allemann, R. and Scrutton, N., Eds, Royal Society of Chemistry, London, UK, Ch. 6, 2008. In Press.
• Yahashiri, A.; Howell, E.E.; Kohen, A. Tuning of the H-Transfer Coordinate in Primitive versus Well-Evolved Enzymes. ChemPhysChem 2008, 9, 980-982 (Communication).
• Bandaria, J.; Dutta, S.; Hill, S.; Kohen, A.; Cheatum, C. Fast Enzyme Dynamics at the Active Site of Formate Dehydrogenase. J. Am. Chem. Soc. 2008, 130, 22-23 (Communication).
• Hong, B.; Maley, F.; Kohen, A. The Role of Y94 in Proton and Hydride Transfers Catalyzed by Thymidylate Synthase. Biochemistry. 2007, 46, 14188-14197.
• Chernyshev, A.; Fleischmann,T.; Koehn, E.M.; Lesley, S.A.; Kohen, A. The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS). Chem. Commun. 2007, 2861-2863
• Kanaan, N.; Marti, S.; Moliner, V.; Kohen, A. A QM/MM Study of the catalytic mechanism of the thymidylate synthase. Biochemistry. 2007, 46, 3704-3713.
• Chernyshev, A.; Fleischmann, T.; Kohen, A. Thymidyl Biosynthesis Enzymes as Antibiotic Targets. Appl. Microbiol. Biotech. 2007, 74, 282-289 (review).
• Kohen, A. Probes for hydrogen tunneling and coupled motion in enzymatic systems, in Hydrogen-Transfer Reactions, Vol. 4. Hynes, Klinman, Limbach, Schowen, Eds., Wiley VCH, Weinheim, 2007, Chapter 12, 1311-1340. [Invited Chapter].
• Wang, L.; Goodey, N.M.; Benkovic, S.J.; Kohen, A. Coordinated Effects of Distal Mutations on Environmentally Coupled Tunneling in Dihydrofolate Reductase. Proc. Natl. Acad. Sci. USA 2006, 103, 15753-15758.
• Markham, K.A.; Kohen, A. Analytical Procedures for the Preparation, Isolation, Analysis and Preservation of Reduced Nicotinamides. 2006, 2, 379-388 (invited review).
• Kohen, A. Kinetic isotope effects as probes for hydrogen tunneling in enzyme catalysis. in Isotope Effects in Chemistry and Biology, Kohen, A. and Limbach, H.H. Eds., Taylor & Francis -CRC Press, Boca Raton, FL, 2006, Ch. 28, pp-743-764. ISBN 0824724496 [Invited Chapter]
• Hong, B.; Haddad, M.; Maley, F.; Jensen, J.H.; Kohen, A. Hydride transfer versus hydrogen radical transfer in thymidylate synthase. J. Am. Chem. Soc. (communication) 2006, 128, 5636 - 5637
• Mason, A.; Agrawal, N.; Washington, T.; Lesley, S.A.; Kohen, A. A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link. Chem. Comm. 2006, 1781 - 1783.
• Limbach, H.H.; Lopez, J.M.; Kohen, A. Arrhenius curves of hydrogen transfers: Tunnel effects, isotope effects and effects of pre-equilibria. Phil. Trans. R. Soc. B, 2006, 361,1399 - 1415.
• Wang, L.; Goodey, N.M.; Benkovic, S.J.; Kohen, A. The role of enzyme dynamics and tunneling in catalyzing hydride transfer: studies of distal mutations of dihydrofolate reductase. Phil. Trans. R. Soc. B, 2006, 361, 1307-1215.
• Wang, L.; Tharp, S.; Seltzer, T.; Benkovic, S.J.; Kohen, A. Effects of a distal mutation on active site chemistry. Biochemistry, 2006, 45, 1383-1392.
• Pu, J.; Ma, S.; Garcia-Viloca, M.; Gao, J.; Truhlar, D.G.; Kohen, A. Nonperfect synchronization of reaction center rehybridization in the transition sate of the hydride transfer catalyzed by dihydrofolate reductase. J. Am. Chem. Soc. 2005, 127, 14789-14886.
• Hong, B; Kohen, A. Microscale synthesis of isotopically labeled 6R-N⁵, N¹⁰ methylene-5, 6, 7, 8-tetrahydrofolate. J. Labeled Comp. Radiopharma. 2005, 48, 759-769.
• Sra, A.K.; Hu, Y.; Martin, G.E.; Snow, D.D.; Ribbe, M.W.; Kohen, A. Competitive 15N Kinetic Isotope Effects of Nitrogenase Catalyzed Dinitrogen Reduction. J. Am. Chem. Soc. 2004, 126, 12768-12769.
• Agrawal, N.; Lesley, S.A.; Kuhn, P.; Kohen, A. Mechanistic studies of a flavin-dependent thymidylate synthase. Biochemistry, 2004, 43, 10295-10301, Accelarated Publication.
• Sikorski, R.S.; Wang,L.; Markham K.A.; Ravi Rajagopalan, P.T.; Benkovic, S.J.; Kohen, A. Tunneling and coupled motion in the E. coli dihydrofolate reductase catalysis. J. Am. Chem. Soc. 2004, 126, 4778-4779.
• Agrawal, N.; Mihai, C.; Kohen, A. Microscale synthesis of isotopically labeled R-[6-XH]-N5,N10-methlene 5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase. Anal. Biochem. 2004, 328, 44-50.
• Agrawal, N.; Hong, B.; Mihai, C.; Kohen, A. Vibrationally enhanced hydrogen tunneling in the E. coli thymidylate synthase catalyzed reaction. Biochemistry, 2004, 43, 1998-2006.
• Markham, K.; Sikorski, R.S.; Kohen, A. Synthesis and utility of 14C-nicotinamide cofactors. Anal. Biochem. 2004, 325, 62-67